Respiratory tract samples

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Endobronchial ultrasound (EBUS) samples

We are one of the few NHS Trusts that provide a consultant led endobronchial ultrasound fine needle aspiration (EBUS FNA) service which started in October 2012.

A Consultant Cyto/histopathologist is on site to provide preliminary diagnoses with technical assistance provided by a biomedical scientist.

This has proved extremely successful in terms of preliminary diagnosis, specimen triage and reflex molecular testing.

EBUS FNA service 1 EBUS FNA service 2

Sputum samples

Best results are achieved with freshly obtained sputa following chest physiotherapy, with an early morning sputum before the patient has eaten.

Contamination with large amounts of saliva or food leads to inadequate specimens.

Multiple specimens (usually x 3) may be necessary, but these should be sent on 3 separate days, not all taken at the same time.

Send in clean, dry container with screw cap.

If examination for eosinophils is required please indicate this on the request form.

Bronchial aspirate/trap/wash/lavage & bronchoalveolar lavage samples

Fresh specimen should be placed in a clean dry container and an equal volume of CytoRich® Red preservative fluid immediately added for fixation.

The time of this fixation should be indicated on the label of the container.

If CytoRich® Red preservative fluid is not available, fresh specimen should be placed in clean dry container. Delay of receipt unfixed samples can lead to deterioration of specimen.

If differential cell count is required, DO NOT USE CytoRich® Red preservative fluid. Please send the sample unfixed.

DO NOT USE FORMALIN FIXATIVE.

fresh specimen

Fresh specimen

cytorich specimen

Specimen with an equal volume of CytoRich® Red preservative fluid

Please note: When the stock of CytoRich® Red preservative fluid is running low or close to its expiry date, please contact the cytology department on 276 5110/5115 for replacement.

Bronchial brush samples

Place brush into clean screw capped container with CytoRich® Red preservative fluid.

Ensure brush is fully immersed in preservative.

The time of this fixation should be indicated on the label of the container.

DO NOT USE FORMALIN FIXATIVE

Please note: When the stock of CytoRich® Red preservative fluid is running low or close to its expiry date, please contact the cytology department on 276 5110/5115 for replacement of stock.

bronchial brush

 

(Last reviewed 27th February 2018)