WGS Sample Requirements

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For Whole Genome Sequencing only fresh tissue can be accepted

Amount of Material

  • Large tumours – 5x5x2mm cube; 10x3x2mm slice; 5mm Punch biopsy
  • Small tumours – 20mm (L) x 2mm (D) core biopsy
  • Biopsy Samples -2-3 needle cores / 1-2 standard endoscopic forceps biopsies

Neoplastic cell content

  • Invasive malignant nuclei ≥30% (<20% necrosis)
  • Tumour assessment to be made on:
    • Frozen section of tissue to be submitted
    • FFPE mirror block of tissue to be submitted
    • Or FFPE of area surrounding punch biopsies to be submitted

Additional information and Transport

  • Do not fix the sample
  • Sample selection should be undertaken within 2 hours of excision. If no please refrigerate.
  • Refrigerated samples should be sent to arrive at the GLH laboratory within 72 hours.
  • If sample is expected to take over 72 hours from excision to arriving at the GLH please freeze the samples prior to dispatch to the GLH using Liquid nitrogen or Cryospray
  • Please refer to local SOP on processing fresh tissue sample for WGS
  • GLH address: North West Genomic Laboratory Hub (Manchester), Manchester Centre for Genomic Medicine, St Mary’s Hospital, Oxford Road, Manchester, M13 9WL