In this section
For Whole Genome Sequencing only fresh tissue can be accepted
Amount of Material
- Large tumours – 5x5x2mm cube; 10x3x2mm slice; 5mm Punch biopsy
- Small tumours – 20mm (L) x 2mm (D) core biopsy
- Biopsy Samples -2-3 needle cores / 1-2 standard endoscopic forceps biopsies
Neoplastic cell content
- Invasive malignant nuclei ≥30% (<20% necrosis)
- Tumour assessment to be made on:
- Frozen section of tissue to be submitted
- FFPE mirror block of tissue to be submitted
- Or FFPE of area surrounding punch biopsies to be submitted
Additional information and Transport
- Do not fix the sample
- Sample selection should be undertaken within 2 hours of excision. If no please refrigerate.
- Refrigerated samples should be sent to arrive at the GLH laboratory within 72 hours.
- If sample is expected to take over 72 hours from excision to arriving at the GLH please freeze the samples prior to dispatch to the GLH using Liquid nitrogen or Cryospray
- Please refer to local SOP on processing fresh tissue sample for WGS
- GLH address: North West Genomic Laboratory Hub (Manchester), Manchester Centre for Genomic Medicine, St Mary’s Hospital, Oxford Road, Manchester, M13 9WL