Non-gynaecological Cytology sample collection

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Serous fluid samples including peritoneal washing

Body cavity fluids (pleural/ascitic/peritoneal/pericardial fluids & peritoneal washing)

30 -100 mls fluid should be sent in a clean dry container with screw cap (Note: no formalin or alcohol should be added to the sample as both of these can cause interference with adherence to slide and quality of staining).

The fluid should be submitted as soon as possible to minimise cell deterioration, so that cell preservation is not compromised.

If there is a delay in delivering the sample to the laboratory, the sample should be kept refrigerated at 4°C (Note – the sample should NOT be frozen).

Serous fluid

Cyst fluids

Cyst fluid should be put into a clean dry container with screw cap.

The fluid should be submitted as soon as possible to minimise cell deterioration, so that cell preservation is not compromised.

If there is a delay in delivering the sample to the laboratory, the sample should be kept refrigerated at 4°C (Note – the sample should NOT be frozen).

Cyst fluids

Gastrointestinal tract brush samples (including bile duct brushes)

  • Place brush into clean screw capped container with CytoRich® Red preservative fluid.Ensure brush is fully immersed in preservative
  • The time of this fixation should be indicated on the label of the container
  • DO NOT USE FORMALIN FIXATIVE
  • Please note: When the stock of CytoRich® Red preservative fluid is running low or close to its expiry date, please contact the cytology department on 276 5110/5115 for replacement of stock.

Urinary tract samples

Urine (including voided, catheter, urethral washings and ileal conduit samples)

Collect urine in a clean, dry container with a screw cap. A 20ml to 50ml container, preferably with yellow lid (below) is suitable

Please do not send urine for cytology in Sarstedt Monovette

An adequate urine sample is the second voided of the day, preferably mid morning or random

Please note: The first sample voided in the morning is unsuitable for cytological analysis

Urine can be collected from catheters as well as washings from the bladder or upper urinary tract. The request form must state the method of collection

If there is a delay in delivering the sample to the laboratory, the urine sample should be kept in a fridge at 4ºC

The preferred method for the collection of industrial urine samples is the same as above (preferably in 25ml Universal tube). Please contact the cytology department in advance when sending large number of samples.

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Cerebrospinal fluid samples

A clean, dry container with screw cap should be use.

CSF samples are liable to degenerate rapidly and as such must be prepared immediately. Please contact the laboratory to inform staff of imminent arrival of a CSF sample, and leave a bleep or contact number.

Latest processing time for samples is 15.30 hrs Mon-Fri. CSF samples must be received at least half an hour before this time.

If out of hours sampling is unavoidable, storing the sample in refrigerator at 4°C may help preserve cells for up to 24 hours.

CSF