Haematinics

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The in-vitro investigations carried out in this laboratory constitute vitamin B12, folate and ferritin assays carried out as part of the diagnosis of anaemia. Intrinsic factor antibody testing (IFAB) for Pernicious Anaemia is carried out on patient samples where the B12 has been shown to be reduced.

Haematinics tests

Here is a list of the haematinics tests carried out by the Haematology department, together with specimen requirements and the estimated time it will take to produce a result.

The results availability times given here represent the time generally required to produce a result following receipt of the sample in the laboratory. Sample transport times are not included.

Where appropriate, if a result is required the same day samples must be received sufficiently early to allow completion of the test. Contact the lab if the test you require is not listed.

These tests are available during the normal working day.

For further advice or information, call 0161 276 4689 or 64803.

(Blood volume and red cell survival in vivo radioisotope studies are carried out by the Department of Nuclear Medicine. Call 0161 276 4820 to arrange in vivo studies.)

Test Specimen requirements Urgent results available (from time of receipt in lab) Non-urgent results available (from time of receipt in lab) Reports available Can be performed at Central site  Can be performed at Trafford
Serum and red cell folate

1 x 4.9ml brown top gel tube

1 x 1.2ml brown top gel tube (paeds only)

N/A 3 working days 5 days Yes Yes
Serum vitamin B12

1 x 4.9ml brown top gel tube

1 x 1.2ml brown top gel tube (paeds only)

N/A

3 working days 5 days Yes Yes

Serum ferritin

1 x 4.9ml brown top gel tube

1 x 1.2ml brown top gel tube (paeds only)

N/A

3 working days

5 days

Yes

 Yes

Serum intrinsic factor antibodies (carried out on patients with a low B12 level – less than 120ng/l)

1 x 4.9ml brown top gel tube

1 x 1.2ml brown top gel tube (paeds only)

N/A

5 working days 7 days Yes No
Erythropoietin (EPO)

1 x 4.9ml brown top gel tube

1 x 1.2ml brown top gel tube (paeds only)

N/A

5 working days 7 days Yes No

Haemochromatosis

C282Y, H63D and S65C genotyping

(See Molecular Diagnostics Centre for more information)

Green cap vacutainer [1.3ml neonate or 2.9ml paediatric and adult] <3 weeks

<3 weeks

Yes

 No

Zinc Protoporphyrin (ZPP)

Paediatric only

1 x 1.2ml red cap EDTA

N/A

3 working days

5 days Yes

No

 

Reference ranges – Haematinics

These reference ranges are to help with interpreting results of Haematinics tests carried out by the Haematology department.

 

Serum folate 3.9 – 20 μg/l
Serum vitamin B12 greater than or equal to 197 ng/l
Serum ferritin

Male 30 – 400 μg/l

Female 13 – 150 μg/l

Erythropoeitin (EPO) 3-18 mIU/mL
Zinc Protoporphyrin (ZPP) Paediatric only 30-80 μmolZPP/mol heam

Factors affecting the results or processing of haematinics and haemolytics tests

Here are some of the factors which could affect the results or processing of haematinics and haemolytics tests. This is not an exhaustive list. There may be other factors affecting the reporting of results which are not listed.

 

Test Factors affecting result/processing Outcome

Red Cell Folate

No EDTA sample

Sent – only clotted sample

Only serum folate can be measured
Red Cell Folate EDTA clotted Would not process
Ferritin, B12, Serum folate

Only EDTA sent

Would not process

Haematinic assays

Haemolysis Would process and add appropriate comment
Haemolytic assays EDTA clotted Would not process
Plasma Viscosity Sample stored in fridge Would not process